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Anti-SUCLG1 Antibody Picoband®, FITC Conjugate

Boster Bio Anti-SUCLG1 Antibody Picoband® catalog # A06274-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial is an enzyme that in humans is encoded by the SUCLG1 gene. This gene encodes the alpha subunit of the heterodimeric enzyme succinate coenzyme A ligase. This enzyme is targeted to the mitochondria and catalyzes the conversion of succinyl CoA and ADP or GDP to succinate and ATP or GTP. Mutations in this gene are the cause of the metabolic disorder fatal infantile lactic acidosis and mitochondrial DNA depletion.

Synonyms

ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL2; HUB

Gene Name

ELAV like RNA binding protein 2

Gene ID

8802

UniProt

P53597

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SUCLG1 recombinant protein (Position: R25-K346).

Clonality

Polyclonal

Tissue Specificity

Brain; neural-specific.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds RNA. Seems to recognize a GAAA motif. Can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Chinopoulos, C., Batzios, S., van den Heuvel, L. P., Rodenburg, R., Smeets, R., Waterham, H. R., Turkenburg, M., Ruiter, J. P., Wanders, R. J. A., Doczi, J., Horvath, G., Dobolyi, A., Vargiami, E., Wevers, R. A., Zafeiriou, D. Mutated SUCLG1 causes mislocalization of SUCLG2 protein, morphological alterations of mitochondria and an early-onset severe neurometabolic disorder. Molec. Genet. Metab. 126: 43-52, 2019. 2. Gross, M. B. Personal Communication. Baltimore, Md. 5/29/2015. 3. James, M., Man, N., Edwards, Y. H., Morris, G. E. The molecular basis for cross-reaction of an anti-dystrophin antibody with alpha-actinin. Biochim. Biophys. Acta 1360: 169-176, 1997.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A06274-1

Product MSDS

https://www.bosterbio.com/msds?sku=A06274-1

Calculated Molecular Weight

49655 MW

Observed Molecular Weight

35 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm.

Protein Name

AP-2 complex subunit mu

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