Anti-SETD3 Antibody Picoband®, FITC Conjugate

Boster Bio Anti-SETD3 Antibody Picoband® catalog # A10965-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SET domain containing 3 (SETD3) is a protein that in humans is encoded by the SETD3 gene. SETD3 is a histone methyltransferase. In mouse muscle cells, Setd3 catalyzes mono- and dimethylation of histone H3 lysine-4 and H3K36 and promotes transcription of genes required for muscle cell differentiation.

Synonyms

Interleukin-17B; IL-17B; Cytokine CX1; Cytokine-like protein ZCYTO7; Neuronal interleukin-17-related factor; Il17b; Nirf, Zcyto7

Gene Name

interleukin 17B

Gene ID

84193

UniProt

Q86TU7

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SETD3 recombinant protein (Position: Q173-R468).

Clonality

Polyclonal

Tissue Specificity

Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Eom, G. H., Kim, K.-B., Kim, J. H., Kim, J.-Y., Kim, J.-R., Kee, H. J., Kim, D.-W., Choe, N., Park, H.-J., Son, H.-J., Choi, S.-Y., Kook, H., Seo, S.-B. Histone methyltransferase SETD3 regulates muscle differentiation. J. Biol. Chem. 286: 34733-34742, 2011. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 2/27/2014. 3. Wilkinson, A. W., Diep, J., Dai, S., Liu, S., Ooi, Y. S., Song, D., Li, T.-M., Horton, J. R., Zhang, X., Liu, C., Trivedi, D. V., Ruppel, K. M., and 10 others. SETD3 is an actin histidine methyltransferase that prevents primary dystocia. Nature 565: 372-376, 2019.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

60 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -