Anti-TTLL2 Antibody Picoband®, FITC Conjugate

Boster Bio Anti-TTLL2 Antibody Picoband® catalog # A15891. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Predicted to enable tubulin binding activity and tubulin-glutamic acid ligase activity. Predicted to be involved in microtubule cytoskeleton organization and protein polyglutamylation. Predicted to be active in cilium.

Synonyms

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

Gene Name

kelch repeat and BTB domain containing 2

Gene ID

83887

UniProt

Q9BWV7

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TTLL2 recombinant protein (Position: A24-E582).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

ELISA,IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ribeiro, M. , Angelini, L. , Roblesribeiro, P. G. , Stelmach, R. , Santos, U. D. P. , & Terrafilho, M. . (2000). Characterization of the human tubulin tyrosine ligase-like 1 gene (ttll1) mapping to 22q13.1. Gene, 257(1), 109-117. 2. Faust, T. B. , Yang, L. , Bacon, C. W. , Jang, G. M. , & Frankel, A. D. . (2018). The hiv-1 tat protein recruits a ubiquitin ligase to reorganize the 7sk snrnp for transcriptional activation. Elife, 7. 3. Ota, T. , Suzuki, Y. , Nishikawa, T. , Otsuki, T. , Sugiyama, T. , & Irie, R. , et al. (2004). Complete sequencing and characterization of 21,243 full-length human cdnas. Nature Genetics.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

24145 MW

Observed Molecular Weight

70 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat<br> Immunofluorescence, 5 μg/ml, Human, Mouse, Rat<br> ELISA, 0.1-0.5 μg/ml, -<br>