Anti-Aanat Antibody Picoband®, FITC Conjugate

Boster Bio Anti-Aanat Antibody Picoband® catalog # A03784-1. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Aralkylamine N-acetyltransferase (AANAT) (EC 2.3.1.87), also known as arylalkylamine N-acetyltransferase or serotonin N-acetyltransferase (SNAT), is an enzyme that is involved in the day/night rhythmic production of melatonin, by modification of serotonin. It is in humans encoded by the ~2.5 kb AANAT gene containing four exons, located on chromosome 17q25. The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternative splicing results in multiple transcript variants.

Synonyms

S-arrestin; 48 kDa protein; Retinal S-antigen; S-AG; Rod photoreceptor arrestin; SAG;

Gene Name

S-antigen visual arrestin

Gene ID

11298

UniProt

O88816

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Aanat recombinant protein (Position: C24-C205).

Clonality

Polyclonal

Tissue Specificity

Retina and pineal gland.

Applications

WB,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated- phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Coon, S. L., del Olmo, E., Young, W. S., III, Klein, D. C. Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87). J. Clin. Endocr. Metab. 87: 4699-4706, 2002. 2. Coon, S. L., Mazuruk, K., Bernard, M., Roseboom, P. H., Klein, D. C., Rodriguez, I. R. The human serotonin N-acetyltransferase (EC 2.3.1.87) gene (AANAT): structure, chromosomal localization, and tissue expression. Genomics 34: 76-84, 1996. 3. Coon, S. L., Roseboom, P. H., Baler, R., Weller, J. L., Namboodiri, M. A. A., Koonin, E. V., Klein, D. C. Pineal serotonin N-acetyltransferase: expression cloning and molecular analysis. Science 270: 1681-1683, 1995.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64133 MW

Observed Molecular Weight

23 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Mouse<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Nucleus, nucleoplasm . Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. These granules are not identical with P bodies or stress granules. .