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Anti-NDUFB8 Antibody Picoband®, FITC Conjugate

Boster Bio Anti-NDUFB8 Antibody Picoband® catalog # A07936-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial is an enzyme that in humans is encoded by the NDUFB8 gene. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Synonyms

Glycerol-3-phosphate dehydrogenase 1-like protein; GPD1-L; GPD1L; KIAA0089

Gene Name

glycerol-3-phosphate dehydrogenase 1 like

Gene ID

4714

UniProt

O95169

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NDUFB8 recombinant protein (Position: M1-I186).

Clonality

Polyclonal

Tissue Specificity

Most highly expressed in heart tissue, with lower levels in the skeletal muscle, kidney, lung and other organs.

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD (H) balance induced by DPD1L.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Dunbar, D. R., Shibasaki, Y., Dobbie, L., Andersson, B., Brookes, A. J. In situ hybridisation mapping of genomic clones for five human respiratory chain complex I genes. Cytogenet. Cell Genet. 78: 21-24, 1997. 2. Emahazion, T., Brookes, A. J. Mapping of the NDUFA2, NDUFA6, NDUFA7, NDUFB8, and NDUFS8 electron transport chain genes by intron based radiation hybrid mapping. Cytogenet. Cell Genet. 82: 114 only, 1998. 3. Loeffen, J. L. C. M., Triepels, R. H., van den Heuvel, L. P., Schuelke, M., Buskens, C. A. F., Smeets, R. J. P., Trijbels, J. M. F., Smeitink, J. A. M. cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed. Biochem. Biophys. Res. Commun. 253: 415-422, 1998.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A07936-1

Product MSDS

https://www.bosterbio.com/msds?sku=A07936-1

Calculated Molecular Weight

49229 MW

Observed Molecular Weight

19-22 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cytoplasm.

Protein Name

Chromaffin granule amine transporter

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