Anti-MEIOB Antibody Picoband®, HRP Conjugate

Boster Bio Anti-MEIOB Antibody Picoband® catalog # A13211-2. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Predicted to enable chromatin binding activity; single-stranded DNA 3'-5' exodeoxyribonuclease activity; and single-stranded DNA binding activity. Predicted to be involved in double-strand break repair via homologous recombination; fertilization; and meiotic nuclear division. Predicted to be located in cytoplasm. Implicated in spermatogenic failure 22.

Synonyms

MEIOB; C16orf73; Meiosis-specific with OB domain-containing protein; EC 3.1.-.-

Gene Name

meiosis specific with OB-fold

Gene ID

254528

UniProt

Q8N635

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human MEIOB recombinant protein (Position: I140-V442). Human MEIOB shares 77.9% and 76.1% amino acid (aa) sequence identity with mouse and rat MEIOB, respectively.

Clonality

Polyclonal

Applications

WB, ICC/IF, FlowCytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Caburet, S., Todeschini, A.-L., Petrillo, C., Martini, E., Farran, N. D., Legois, B., Livera, G., Younis, J. S., Shalev, S., Veitia, R. A. A truncating MEIOB mutation responsible for familial primary ovarian insufficiency abolishes its interaction with its partner SPATA22 and their recruitment to DNA double-strand breaks. EBioMedicine 42: 524-531, 2019. 2. Gershoni, M., Hauser, R., Yogev, L., Lehavi, O., Azem, F., Yavetz, H., Pietrokovski, S., Kleiman, S. E. A familial study of azoospermic men identifies three novel causative mutations in three new human azoospermia genes. Genet. Med. 19: 998-1006, 2017. 3. Luo, M., Yang, F., Leu, N. A., Landaiche, J., Handel, M. A., Benavente, R., La Salle, S., Wang, P. J. MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination. Nature Commun. 4: 2788, 2013. Note: Electronic Article.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

49 kDa

Observed Molecular Weight

49 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, Human