Anti-C19orf10/MYDGF Antibody Picoband®, HRP Conjugate

Boster Bio Anti-C19orf10/MYDGF Antibody Picoband® catalog # A08602. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted) bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis.

Synonyms

Gap junction gamma-1 protein;Connexin-45;Cx45;Gap junction alpha-7 protein;GJC1;GJA7;

Gene Name

Gap junction gamma-1 protein

Gene ID

56005

UniProt

Q969H8

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human C19orf10/MYDGF recombinant protein (Position: V32-L173).

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Tulin, E. E., Onoda, N., Nakata, Y., Maeda, M., Hasegawa, M., Nomura, H., Kitamura, T. SF20/IL-25, a novel bone marrow stroma-derived growth factor that binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation. J. Immun. 167: 6338-6347, 2001. Note: Retraction: J. Immun. 170: 1593 only, 2003. 2. Wang, P., Mariman, E., Keijer, J., Bouwman, F., Noben, J.-P., Robben, J., Renes, J. Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines. Cell. Molec. Life Sci. 61: 2405-2417, 2004. 3. Weiler, T., Du, Q., Krokhin, O., Ens, W., Standing, K., El-Gabalawy, H., Wilkins, J. A. The identification and characterization of a novel protein, c19orf10, in the synovium. Arthritis Res. Ther. 9: R30, 2007. Note: Electronic Article.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

45470 MW

Observed Molecular Weight

15 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -