Anti-NSG1 Antibody Picoband®, HRP Conjugate

Boster Bio Anti-NSG1 Antibody Picoband® catalog # A12107-1. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

NSG1, also known as Neuron-specific protein family member 1, contains an N-terminal nuclear localization motif and a central hydrophobic segment. NSG1 localizes to the nucleus in mitotic neuronal cell cultures and to the cytoplasm in postmitotic neurons. Mouse and human NSG1 share 97% sequence identity and they differs from each other by only 5 amino acid residues. The protein is expressed in all regions of rat brain. In hamster, it was preferentially expressed in adult brain.GenScript Rabbit Anti-NSG1 Polyclonal Antibody is developed in rabbit using purified His-tagged human NSG1 full length recombinant protein.

Synonyms

Negative elongation factor B; NELF-B; Cofactor of BRCA1; NELFB; COBRA1; KIAA1182

Gene Name

negative elongation factor complex member B

Gene ID

27065

UniProt

P42857

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NSG1 recombinant protein (Position: M1-Q176).

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Applications

ELISA,IHC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Carlock, L., Vo, T., Lorincz, M., Walker, P. D., Bessert, D., Wisniewski, D., Dunbar, J. C. Variable subcellular localization of a neuron-specific protein during NTera 2 differentiation into post-mitotic human neurons. Molec. Brain Res. 42: 202-212, 1996. 2. Carlock, L., Wisniewski, D., Lorincz, M., Pandrangi, A., Vo, T. An estimate of the number of genes in the Huntington disease gene region and the identification of 13 transcripts in the 4p16.3 segment. Genomics 13: 1108-1118, 1992.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

21 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -