Anti-RPH3A Antibody Picoband®, HRP Conjugate

Boster Bio Anti-RPH3A Antibody Picoband® catalog # A10247-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Rabphilin-3A is a protein that in humans is encoded by the RPH3A gene. The protein encoded by this gene is thought to be an effector for RAB3A, which is a small G protein that acts in the late stages of neurotransmitter exocytosis. The encoded protein may be involved in neurotransmitter release and synaptic vesicle traffic.

Synonyms

Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA

Gene Name

pre T cell antigen receptor alpha

Gene ID

22895

UniProt

Q9Y2J0

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RPH3A recombinant protein (Position: R78-Q564).

Clonality

Polyclonal

Tissue Specificity

Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Applications

WB,IHC,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Inagaki, N., Mizuta, M., Seino, S. Cloning of a mouse rabphilin-3A expressed in hormone-secreting cells. J. Biochem. 116: 239-242, 1994. 2. Lin, C.-C., Huang, C.-C., Lin, K.-H., Cheng, K.-H., Yang, D.-M., Tsai, Y.-S., Ong, R.-Y., Huang, Y.-N., Kao, L.-S. Visualization of Rab3A dissociation during exocytosis: a study by total internal reflection microscopy. J. Cell. Physiol. 211: 316-326, 2007. 3. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 63-70, 1999.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

41409 MW

Observed Molecular Weight

85 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat<br> ELISA, 0.1-0.5 μg/ml, -<br>