Anti-SCFD2 Antibody Picoband®, HRP Conjugate

Boster Bio Anti-SCFD2 Antibody Picoband® catalog # A15482-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SCFD2 (sec1 family domain containing 2), also known as STXBP1L1 (syntaxinbinding protein 1-like 1), is a 684 amino acid protein suggested to play a role in protein transport. Existing as two alternatively spliced isoforms, SCFD2 is a member of the STXBP/unc-18/SEC1 family and is encoded by a gene that maps to human chromosome 4q12. Chromosome 4 represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington’s disease, is on chromosome 4. FGFR-3 is also encoded on chromosome 4 and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease

Synonyms

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

Gene Name

kelch repeat and BTB domain containing 2

Gene ID

152579

UniProt

Q8WU76

Host

Rabbit

Reactivity

Human,Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SCFD2 recombinant protein (Position: K20-H585).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Mitobe, Y. , Iino, K. , Takayama, K. , Ikeda, K. , Suzuki, T. , & Aogi, K. , et al. (2020). Psf promotes er-positive breast cancer progression via posttranscriptional regulation of esr1 and scfd2. Cancer research, 80(11), 2230-2242. 2. Takeiwa, T. , Ikeda, K. , Suzuki, T. , Sato, W. , Iino, K. , & Mitobe, Y. , et al. (2022). Pspc1 is a potential prognostic marker for hormone-dependent breast cancer patients and modulates rna processing of esr1 and scfd2. Scientific reports, 12(1), 9495. 3. (2010). Genome-wide association identifies atoh7 as a major gene determining human optic disc size. Human Molecular Genetics, 19(13), 2716.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

24145 MW

Observed Molecular Weight

75 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -