Anti-PPM1G Antibody Picoband®, HRP Conjugate

Boster Bio Anti-PPM1G Antibody Picoband® catalog # A06725-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Protein phosphatase 1G is an enzyme that in humans is encoded by the PPM1G gene. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression.

Synonyms

Neurocan core protein; Chondroitin sulfate proteoglycan 3; NCAN; CSPG3, NEUR;

Gene Name

neurocan

Gene ID

63977

UniProt

O15355

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PPM1G recombinant protein (Position: E48-Q457).

Clonality

Polyclonal

Tissue Specificity

Brain.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Gross, M. B. Personal Communication. Baltimore, Md. 9/30/2014. 2. Murray, M. V., Kobayashi, R., Krainer, A. R. The type 2C Ser/Thr phosphatase PP2C-gamma is a pre-mRNA splicing factor. Genes Dev. 13: 87-97, 1999. 3. Travis, S. M., Welsh, M. J. PP2C-gamma: a human protein phosphatase with a unique acidic domain. FEBS Lett. 412: 415-419, 1997.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

104553 MW

Observed Molecular Weight

75 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -