Anti-POLR2H Antibody Picoband®, HRP Conjugate

Boster Bio Anti-POLR2H Antibody Picoband® catalog # A11270-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

DNA-ed RNA polymerases I, II, and III subunit RPABC3 is a protein that in humans is encoded by the POLR2H gene. The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-ed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.

Synonyms

Interleukin-17B; IL-17B; Cytokine CX1; Cytokine-like protein ZCYTO7; Neuronal interleukin-17-related factor; Il17b; Nirf, Zcyto7

Gene Name

interleukin 17B

Gene ID

5437

UniProt

P52434

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human POLR2H recombinant protein (Position: D38-F150).

Clonality

Polyclonal

Tissue Specificity

Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. McKune, K., Moore, P. A., Hull, M. W., Woychik, N. A. Six human RNA polymerase subunits functionally substitute for their yeast counterparts. Molec. Cell. Biol. 15: 6895-6900, 1995. 2. Shpakovski, G. V., Acker, J., Wintzerith, M., Lacroix, J. F., Thuriaux, P., Vigneron, M. Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae. Molec. Cell. Biol. 15: 4702-4710, 1995.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

17 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -