Anti-TMEM87A Antibody Picoband®, HRP Conjugate

Boster Bio Anti-TMEM87A Antibody Picoband® catalog # A14716-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Involved in retrograde transport, endosome to Golgi. Located in Golgi cisterna membrane.

Synonyms

Transmembrane protein 199; TMEM199; C17orf32

Gene Name

transmembrane protein 199

Gene ID

25963

UniProt

Q8NBN3

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TMEM87A recombinant protein (Position: D38-E555).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Accessory component of the proton-transporting vacuolar (V)-ATPase protein pump involved in intracellular iron homeostasis. In aerobic conditions, required for intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. Necessary for endolysosomal acidification and lysosomal degradation. May be involved in Golgi homeostasis.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Patkunarajah, A. , Stear, J. H. , Moroni, M. , Schroeter, L. , & Poole, K. . (2020). Tmem87a/elkin1, a component of a novel mechanoelectrical transduction pathway, modulates melanoma adhesion and migration. eLife Sciences Publications, Ltd. 2. Hirata, T. , Fujita, M. , Nakamura, S. , Gotoh, K. , Motooka, D. , & Murakami, Y. , et al. (2015). Post-golgi anterograde transport requires garp-dependent endosome-to-tgn retrograde transport. Molecular Biology of the Cell, 3071-3084. 3. Christian, S. L. , Fantes, J. A. , Mewborn, S. K. , Bing, H. , & Ledbetter, D. H. . Large genomic duplicons map to sites of instability in the prader-willi/angelman syndrome chromosome region (15q11-q13). Human Molecular Genetics(6), 1025-1037.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64099 MW

Observed Molecular Weight

70 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -

Subcellular Location

Endoplasmic reticulum membrane. Multi-pass membrane protein. COPI-coated vesicle membrane. Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Multi-pass membrane protein.