Anti-Hfe Antibody Picoband®, HRP Conjugate

Boster Bio Anti-Hfe Antibody Picoband® catalog # A00506-5. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Human homeostatic iron regulator protein, also known as the HFE protein (High FE2+), is a protein which in humans is encoded by the HFE gene. Enables transferrin receptor binding activity. Involved in positive regulation of gene expression. Acts upstream of or within hormone biosynthetic process and multicellular organismal iron ion homeostasis. Part of HFE-transferrin receptor complex. Is expressed in brain; choroid invagination; diencephalon roof plate; medulla oblongata part of 4th ventricle choroid plexus; and metencephalon part of 4th ventricle choroid plexus. Used to study hemochromatosis type 1. Human ortholog(s) of this gene implicated in several diseases, including acute porphyria (multiple); arthritis (multiple); bone marrow cancer (multiple); hemochromatosis (multiple); and liver disease (multiple). Orthologous to human HFE (homeostatic iron regulator).

Synonyms

Hereditary hemochromatosis protein;HLA-H;HFE;HLAH;

Gene Name

Hereditary hemochromatosis protein

Gene ID

15216

UniProt

P70387

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Hfe recombinant protein (Position: N112-H227).

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues tested except brain.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Aguilar-Martinez, P., Bismuth, M., Picot, M. C., Thelcide, C., Pageaux, G.-P., Blanc, F., Blanc, P., Schved, J.-F., Larrey, D. Variable phenotypic presentation of iron overload in H63D homozygotes: are genetic modifiers the cause? Gut 48: 836-842, 2001. 2. Allen, K. J., Gurrin, L. C., Constantine, C. C., Osborne, N. J., Delatycki, M. B., Nicoll, A. J., McLaren, C. E., Bahlo, M., Nisselle, A. E., Vulpe, C. D., Anderson, G. J., Southey, M. C., Giles, G. G., English, D. R., Hopper, J. L., Olynyk, J. K., Powell, L. W., Gertig, D. M. Iron-overload-related disease in HFE hereditary hemochromatosis. New Eng. J. Med. 358: 221-230, 2008. 3. Andersen, R. V., Tybjaerg-Hansen, A., Appleyard, M., Birgens, H., Nordestgaard, B. G. Hemochromatosis mutations in the general population: iron overload progression rate. Blood 103: 2914-2919, 2004.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

40108 MW

Observed Molecular Weight

40-58 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse ELISA, 0.1-0.5 μg/ml, -