Anti-TIP49A/RUVBL1 Antibody Picoband®, HRP Conjugate

Boster Bio Anti-TIP49A/RUVBL1 Antibody Picoband® catalog # A02049-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

RuvB-like 1 (E. coli), also known as RUVBL1 and TIP49, is a human gene. This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.

Synonyms

G2/mitotic-specific cyclin-B2; CCNB2

Gene Name

cyclin B2

Gene ID

8607

UniProt

Q9Y265

Host

Rabbit

Reactivity

Human,Monkey,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TIP49A/RUVBL1 recombinant protein (Position: Q13-D23).

Clonality

Polyclonal

Tissue Specificity

Strongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Expressed in endothelial cells. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Bauer, A., Huber, O., Kemler, R. Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein. Proc. Nat. Acad. Sci. 95: 14787-14792, 1998. 2. Doyon, Y., Selleck, W., Lane, W. S., Tan, S., Cote, J. Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans. Molec. Cell. Biol. 24: 1884-1896, 2004. 3. Fazzio, T. G., Huff, J. T., Panning, B. An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity. Cell 134: 162-174, 2008.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

37492 MW

Observed Molecular Weight

54 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -