Anti-FHL1 Antibody Picoband®, HRP Conjugate

Boster Bio Anti-FHL1 Antibody Picoband® catalog # A01258-3. Tested in ELISA, WB applications. This antibody reacts with Human， Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Four and a half LIM domains protein 1 is a protein that in humans is encoded by the FHL1 gene. This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.

Synonyms

Four and a half LIM domains protein 1; FHL-1; KyoT; RBP-associated molecule 14-1; RAM14-1; Skeletal muscle LIM-protein 1; SLIM; SLIM-1

Gene Name

four and a half LIM domains 1

Gene ID

2273

UniProt

Q13642

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human FHL1 recombinant protein (Position: K133-Q179).

Clonality

Polyclonal

Tissue Specificity

Isoform 1 seems to be most abundant in each tissue and isoform 2 much less abundant. Isoform 1 is highly expressed in skeletal muscle and lung, and to a lesser extent in heart, brain and kidney. Isoform 2 was found in brain, lung kidney and genital organs.

Applications

WB,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Brown, S., McGrath, M. J., Ooms, L. M., Gurung, R., Maimone, M. M., Mitchell, C. A. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1: localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication. J. Biol. Chem. 274: 27083-27091, 1999. 2. Emmanuele, V., Kubota, A., Garcia-Diaz, B., Garone, C., Akman, H. O., Sanchez-Gutierrez, D., Escudero, L. M., Kariya, S., Homma, S., Tanji, K., Quinzii, C. M., Hirano, M. Fhl1 W122S causes loss of protein function and late-onset mild myopathy. Hum. Molec. Genet. 24: 714-726, 2015. 3. Fimia, G. M., De Cesare, D., Sassone-Corsi, P. A family of LIM-only transcriptional coactivators: tissue-specific expression and selective activation of CREB and CREM. Molec. Cell. Biol. 20: 8613-8622, 2000.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.