Anti-Golgin 97/GOLGA1 Antibody Picoband®, HRP Conjugate

Boster Bio Anti-Golgin 97/GOLGA1 Antibody Picoband® catalog # A13524-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.

Synonyms

RNA-binding protein 47;RNA-binding motif protein 47;RBM47;

Gene Name

RNA-binding protein 47

Gene ID

2800

UniProt

Q92805

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Golgin 97/GOLGA1 recombinant protein (Position: M1-K752).

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Griffith, K. J., Chan, E. K. L., Lung, C.-C., Hamel, J. C., Guo, X., Miyachi, K., Fritzler, M. J. Molecular cloning of a novel 97-Kd Golgi complex autoantigen associated with Sjogren's syndrome. Arthritis Rheum. 40: 1693-1702, 1997. 2. Lu, L., Tai, G., Hong, W. Autoantigen golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Molec. Biol. Cell 15: 4426-4443, 2004. 3. Wong, M., Munro, S. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science 346: 1256898, 2014. Note: Electronic Article.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64099 MW

Observed Molecular Weight

88-97 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -