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Anti-SDS Antibody Picoband®, HRP Conjugate

Boster Bio Anti-SDS Antibody Picoband® catalog # A02273-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Serine dehydratase or L-serine ammonia lyase (SDH) is in the β-family of pyridoxal phosphate-dependent (PLP) enzymes. This gene encodes one of three enzymes that are involved in metabolizing serine and glycine. L-serine dehydratase converts L-serine to pyruvate and ammonia and requires pyridoxal phosphate as a cofactor. The encoded protein can also metabolize threonine to NH4+ and 2-ketobutyrate. The encoded protein is found predominantly in the liver.

Synonyms

Mitochondrial uncoupling protein 2;UCP 2;Solute carrier family 25 member 8;UCPH;UCP2;SLC25A8;

Gene Name

Mitochondrial uncoupling protein 2

Gene ID

10993

UniProt

P20132

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SDS recombinant protein (Position: M1-K328).

Clonality

Polyclonal

Tissue Specificity

Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ogawa, H., Gomi, T., Konishi, K., Date, T., Nakashima, H., Nose, K., Matsuda, Y., Peraino, C., Pitot, H. C., Fujioka, M. Human liver serine dehydratase: cDNA cloning and sequence homology with hydroxyamino acid dehydratases from other sources. J. Biol. Chem. 264: 15818-15823, 1989. [PubMed: 2674117, related citations] [Full Ogawa, H., Gomi, T., Konishi, K., Date, T., Nakashima, H., Nose, K., Matsuda, Y., Peraino, C., Pitot, H. C., Fujioka, M. Human liver serine dehydratase: cDNA cloning and sequence homology with hydroxyamino acid dehydratases from other sources. J. Biol. Chem. 264: 15818-15823, 1989.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

33229 MW

Observed Molecular Weight

42 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein.

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