Anti-ERAP2 Antibody Picoband®, HRP Conjugate

Boster Bio Anti-ERAP2 Antibody Picoband® catalog # A04269-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Endoplasmic reticulum aminopeptidase 2 is a protein that in humans is encoded by the ERAP2 gene. This gene encodes a zinc metalloaminopeptidase of the M1 protease family that resides in the endoplasmic reticulum and functions in N-terminal trimming antigenic epitopes for presentation by major histocompatibility complex (MHC) class I molecules. Certain mutations in this gene are associated with the inflammatory arthritis syndrome ankylosing spondylitis and pre-eclampsia. This gene is located adjacent to a closely related aminopeptidase gene on chromosome 5.

Synonyms

Calretinin; CR; 29 kDa calbindin; CALB2; CAB29

Gene Name

calbindin 2

Gene ID

64167

UniProt

Q6P179

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ERAP2 recombinant protein (Position: K13-T960).

Clonality

Polyclonal

Tissue Specificity

Brain.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

References & Citations

1. Cagliani, R., Riva, S., Biasin, M., Fumagalli, M., Pozzoli, U., Lo Caputo, S., Mazzotta, F., Piacentini, L., Bresolin, N., Clerici, M., Sironi, M. Genetic diversity at endoplasmic reticulum aminopeptidases is maintained by balancing selection and is associated with natural resistance to HIV-1 infection. Hum. Molec. Genet. 19: 4705-4714, 2010. 2. Saveanu, L., Carroll, O., Lindo, V., Del Val, M., Lopez, D., Lepelletier, Y., Greer, F., Schomburg, L., Fruci, D., Niedermann, G., van Endert, P. M. Concerted peptide trimming by human ERAP1 and ERAP2 aminopeptidase complexes in the endoplasmic reticulum. Nature Immun. 6: 689-697, 2005. 3. Tanioka, T., Hattori, A., Masuda, S., Nomura, Y., Nakayama, H., Mizutani, S., Tsujimoto, M. Human leukocyte-derived arginine aminopeptidase: the third member of the oxytocinase subfamily of aminopeptidases. J. Biol. Chem. 278: 32275-32283, 2003.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

110 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -

Subcellular Location

Cuticular plate. Cytosol. Nucleus. Synaptic membrane. Dendrite. Gap junction. Neuron projection. Parallel fiber to Purkinje cell synapse. Presynapse. Stereocilium. Synapse.