Anti-Tspan1 Antibody Picoband®, HRP Conjugate

Boster Bio Anti-Tspan1 Antibody Picoband® catalog # A08242-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

TSPAN1 belongs to the large family of tetraspanins, so named because they contain 4 transmembrane domains. Tetraspanins are frequently expressed at the cell surface in association with each other and with other molecules, such as integrins (see ITGB1; 135630), and they function to regulate cell adhesion, migration, proliferation, and differentiation.

Synonyms

Transforming growth factor beta activator LRRC32; Garpin; Glycoprotein A repetitions predominant; GARP; Leucine-rich repeat-containing protein 32; LRRC32; D11S833E

Gene Name

leucine rich repeat containing 32

Gene ID

298436

UniProt

Q6AYR9

Host

Rabbit

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived rat Tspan1 recombinant protein (Position: A125-A212).

Clonality

Polyclonal

Tissue Specificity

Heart, placenta, skeletal muscle, kidney, lung and pancreas.

Applications

ELISA,Flow Cytometry,IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta. Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity).

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

References & Citations

1. Serru, V., Dessen, P., Boucheix, C., Rubinstein, E. Sequence and expression of seven new tetraspans. Biochim. Biophys. Acta 1478: 159-163, 2000.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

28-30 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat Immunofluorescence, 5 μg/ml, Rat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Rat ELISA, 0.1-0.5μg/ml,