Anti-DNAJC10 Antibody Picoband®, HRP Conjugate

Boster Bio Anti-DNAJC10 Antibody Picoband® catalog # A07954-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

DnaJ homolog subfamily C member 10 is a protein that in humans is encoded by the DNAJC10 gene. This gene encodes an endoplasmic reticulum co-chaperone which is part of the endoplasmic reticulum-associated degradation complex involved in recognizing and degrading misfolded proteins. The encoded protein reduces incorrect disulfide bonds in misfolded glycoproteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Synonyms

DAZ-associated protein 1; Deleted in azoospermia-associated protein 1; DAZAP1

Gene Name

DAZ associated protein 1

Gene ID

54431

UniProt

Q8IXB1

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DNAJC10 recombinant protein (Position: D33-L793).

Clonality

Polyclonal

Tissue Specificity

Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

RNA-binding protein, which may be required during spermatogenesis.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Cunnea, P. M., Miranda-Vizuete, A., Bertoli, G., Simmen, T., Damdimopoulos, A. E., Hermann, S., Leinonen, S., Huikko, M. P., Gustafsson, J.-A., Sitia, R., Spyrou, G. ERdj5, an endoplasmic reticulum (ER)-resident protein containing DnaJ and thioredoxin domains, is expressed in secretory cells or following ER stress. J. Biol. Chem. 278: 1059-1066, 2003. 2. Hosoda, A., Kimata, Y., Tsuru, A., Kohno, K. JPDI, a novel endoplasmic reticulum-resident protein containing both a BiP-interacting J-domain and thioredoxin-like motifs. J. Biol. Chem. 278: 2669-2676, 2003. 3. Ushioda, R., Hoseki, J., Araki, K., Jansen, G., Thomas, D. Y., Nagata, K. ERdj5 is required as a disulfide reductase for degradation of misfolded proteins in the ER. Science 321: 569-572, 2008

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

49229 MW

Observed Molecular Weight

91 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -