Anti-Glypican 5/GPC5 Antibody Picoband®, HRP Conjugate

Boster Bio Anti-Glypican 5/GPC5 Antibody Picoband® catalog # A07532-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Synonyms

Formin-1;Limb deformity protein homolog;FMN1;FMN, LD;

Gene Name

Formin-1

Gene ID

2262

UniProt

P78333

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Glypican 5/GPC5 recombinant protein (Position: Q28-D538).

Clonality

Polyclonal

Tissue Specificity

Expressed ubiquitously.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a role in the formation of adherens junction and the polymerization of linear actin cables. .

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Neat, M. J., Foot, N., Jenner, M., Goff, L., Ashcroft, K., Burford, D., Dunham, A., Norton, A., Lister, T. A., Fitzgibbon, J. Localisation of a novel region of recurrent amplification in follicular lymphoma to an ~6.8 Mb region of 13q32-33. Genes Chromosomes Cancer 32: 236-243, 2001. 2. Veugelers, M., Vermeesch, J., Reekmans, G., Steinfeld, R., Marynen, P., David, G. Characterization of glypican-5 and chromosomal localization of human GPC5, a new member of the glypican gene family. Genomics 40: 24-30, 1997. 3. Yu, W., Inoue, J., Imoto, I., Matsuo, Y., Karpas, A., Inazawa, J. GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma cell lines. J. Hum. Genet. 48: 331-335, 2003.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

157578 MW

Observed Molecular Weight

64 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 4μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -

Subcellular Location

Nucleus . Cytoplasm . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Localization to the adherens junctions is alpha-catenin-dependent. Also localizes to F-actin bundles originating from adherens junctions and to microtubules (By similarity). .