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Recombinant Sparus aurata Fibroblast growth factor (fgf2) (Active)

This Recombinant Sparus aurata Fibroblast growth factor (fgf2) (Active) spans the amino acid sequence from region 1-155aa. Purity: Greater than 95% as determined by SDS-PAGE.

Product Specifications

Product Name Alternative

Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB

UniProt

A0A671VAT8

Expression Region

1-155aa

Tag

Tag free

Source

E.coli

Origin

Sparus aurata (Gilthead sea bream)

Field of Research

Cardiovascular Research; Stem Cell & Developmental Biology

Endotoxin

≤200 EU/mg by the LAL method

Purity

Greater than 95% as determined by SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤2.0 ng/mL.

Form

Lyophilized powder

Molecular Weight

17.1 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Preservative

Lyophilized from a 0.2 μm filtered solution containing 10 mM PB, 250 mMNaCl, 5% mannitol and 0.01% Tween 80, pH 7.4

AA Sequence

MATGEITTLPSTPEDGGSGGFPPGSFKEPKRLYCKNGGFFLRIKSDGGVDGIREKNDPHIKLQLQATSVGEVVIKGVCANRYLAMNRDGRLFGARRATDECHFLERLESNNYNTYRSRKYPNMYVALKRTGQYKSGSKTGPGQKAILFLPMSAKC

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