Bovine IgG
Bovine IgG
Product Specifications
Product Name Alternative
Bovine IgG whole molecule, Bovine Immunoglobulin G, cow IgG
Conjugation
Unconjugated
Source
Bovine
Origin
Bovine
Field of Research
Protein Biochemistry
Concentration
10.0 mg/mL
Dilution
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Purity
Bovine IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Bovine IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum and anti-Bovine IgG.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Bovine IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Bovine IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 5.0 mL
Tested Applications
SDS-PAGE
Preservative
Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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