Mouse IgG
Mouse IgG
Product Specifications
Product Name Alternative
Immunoglobulin G from Mouse, IgG from Mouse, purified polyclonal IgG antibody
Conjugation
Unconjugated
Source
Mouse
Origin
Mouse
Field of Research
Protein Biochemistry
Concentration
10.0 mg/mL
Dilution
ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IP: User Optimized, WB: User Optimized
Purity
Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
Form
Lyophilized
Storage Conditions
Store purified Mouse IgG at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Mouse IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 2.5 mL
Tested Applications
SDS-PAGE
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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