Anti-CSEN/KCNIP3 Antibody Picoband®, PE Conjugate

Boster Bio Anti-CSEN/KCNIP3 Antibody catalog # PA2272. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

KCNIP3, also known as Calsenilin, is a calcium-dependent transcriptional repressor that is highly expressed in central nervous system, thyroid gland, testis, thymus, and immune cells. This gene encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins, which belong to the neuronal calcium sensor family of proteins. KCNIP3 is mapped to 2q11.1. The encoded protein functions as a calcium-regulated transcriptional repressor, and interacts with presenilins. KCNIP3-regulated genes have a critical function in Ig subclass production and proliferation in B lymphocytes. KCNIP3 has been shown to interact with PSEN1 and PSEN2.

Synonyms

Calsenilin;A-type potassium channel modulatory protein 3;DRE-antagonist modulator;DREAM;Kv channel-interacting protein 3;KChIP3;KCNIP3;CSEN, DREAM, KCHIP3;

Gene Name

Calsenilin

UniProt

Q9Y2W7

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CSEN.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease. .

Applications

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity). .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Carrion AM, Link WA, Ledo F, Mellstrom B, Naranjo JR (March 1999). "DREAM is a Ca2+-regulated transcriptional repressor".Nature 398 (6722): 80–4. 2. "Entrez Gene: KCNIP3 Kv channel interacting protein 3, calsenilin" 3. Savignac, M., Mellstrom, B., Bebin, A.-G., Oliveros, J. C., Delpy, L., Pinaud, E., Naranjo, J. R. Increased B cell proliferation and reduced Ig production in DREAM transgenic mice. J. Immun. 185: 7527-7536, 2010.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29231 MW

Observed Molecular Weight

29 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Cytoplasm . Cell membrane ; Lipid-anchor . Endoplasmic reticulum . Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus. .