Anti-EAAT1/SLC1A3 Antibody Picoband®, PE Conjugate

Boster Bio Anti-EAAT1/SLC1A3 Antibody catalog # PA2185. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. SLC1A3 is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH). This gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. This gene acts as a symport by cotransporting sodium.

Synonyms

Excitatory amino acid transporter 1;Sodium-dependent glutamate/aspartate transporter 1;GLAST-1;Solute carrier family 1 member 3;SLC1A3;EAAT1, GLAST, GLAST1;

Gene Name

Excitatory amino acid transporter 1

UniProt

P43003

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1, different from the related rat and mouse sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Applications

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. de Vries, B., Mamsa, H., Stam, A. H., Wan, J., Bakker, S. L. M., Vanmolkot, K. R. J., Haan, J., Terwindt, G. M., Boon, E. M. J., Howard, B. D., Frants, R. R., Baloh, R. W., Ferrari, M. D., Jen, J. C., van den Maagdenberg, A. M. J. M. Episodic ataxia associated with EAAT1 mutation C186S affecting glutamate reuptake. Arch. Neurol. 66: 97-101, 2009. Note: Errata: Arch. Neurol. 66: 497 only, 2009; Arch. Neurol. 66: 772 only, 2009. 2. Matsugami, T. R., Tanemura, K., Mieda, M., Nakatomi, R., Yamada, K., Kondo, T., Ogawa, M., Obata, K., Watanabe, M., Hashikawa, T., Tanaka, K. Indispensability of the glutamate transporters GLAST and GLT1 to brain development. Proc. Nat. Acad. Sci. 103: 12161-12166, 2006.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

59572 MW

Observed Molecular Weight

274 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, By Heat <br>Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human<br>Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human<br>