Anti-Caspase-12/CASP12 Antibody Picoband®, PE Conjugate

Boster Bio Anti-Caspase-12/CASP12 Antibody catalog # PA2103. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The CASP12 gene was mapped to chromosome 11q22.3. Csp12L attenuates the inflammatory and innate immune response to endotoxins and in doing so may constitute a risk factor for developing sepsis. The estrogen-mediated inhibition of CSP12L expression is a built-in mechanism that has evolved to protect females from infection by favoring a more robust inflammatory response to pathogens, perhaps notably in childbirth.

Synonyms

Inactive caspase-12;CASP-12;CASP12;UNQ9415/PRO34398;

Gene Name

Inactive caspase-12

UniProt

Q6UXS9

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-12.

Clonality

Polyclonal

Tissue Specificity

Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Fischer, H., Koenig, U., Eckhart, L., Tschachler, E. Human caspase 12 has acquired deleterious mutations. Biochem. Biophys. Res. Commun. 293: 722-726, 2002. 2. Saleh, M., Mathison, J. C., Wolinski, M. K., Bensinger, S. J., Fitzgerald, P., Droin, N., Ulevitch, R. J., Green D. R., Nicholson, D. W. Enhanced bacterial clearance and sepsis resistance in caspase-12-deficient mice. Nature 440: 1064-1068, 2006. 3. Yeretssian, G., Doiron, K., Shao, W., Leavitt, B. R., Hayden, M. R., Nicholson, D. W., Saleh, M. Gender differences in expression of the human caspase-12 long variant determines susceptibility to Listeria monocytogenes infection. Proc. Nat. Acad. Sci. 106: 9016-9020, 2009.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.