Anti-Dopamine Receptor D3/DRD3 Antibody Picoband®, PE Conjugate

Boster Bio Anti-Dopamine Receptor D3/DRD3 Antibody catalog # PA1584. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

DRD3 is a dopamine receptor that differs in its pharmacology and signaling system from the D1 and D2 receptors and represents both an autoreceptor and a postsynaptic receptor. DRD3 is localized to limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions. The DRD3 gene, which is mapped to chromosome 3, is like the DRD2 gene but unlike most other members of this superfamily and it contains introns, 5 in number. The position of 2 of the introns corresponds to that of introns in DRD2. The D3 receptor appeared to mediate some of the effects of antipsychotic drugs and drugs used in the treatment of Parkinson disease, which were previously though to interact only with D2 receptors.

Synonyms

D (3) dopamine receptor;Dopamine D3 receptor;DRD3;

Gene Name

D(3) dopamine receptor

UniProt

P35462

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Dopamine Receptor D3, different from the related rat and mouse sequences by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Brain.

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Sokoloff, P., Giros, B., Martres, M.-P., Bouthenet, M.-L., Schwartz, J.-C.Molecular cloning and characterization of a novel dopamine receptor (D-3) as a target for neuroleptics.Nature 347: 146-151, 1990. 2. Le Coniat, M., Sokoloff, P., Hillion, J., Martres, M.-P., Giros, B., Pilon, C., Schwartz, J.-C., Berger, R.Chromosomal localization of the human D-3 dopamine receptor gene.Hum. Genet. 87: 618-620, 1991. 3. Sokoloff, P., Giros, B., Martres, M.-P., Bouthenet, M.-L., Schwartz, J.-C.Molecular cloning and characterization of a novel dopamine receptor (D-3) as a target for neuroleptics.Nature 347: 146-151, 1990.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44225 MW

Observed Molecular Weight

42 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat<br>Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse<br>

Subcellular Location

Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells.