Anti-GDNF Antibody Picoband®, PE Conjugate

Boster Bio Anti-GDNF Antibody catalog # PA1465. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands (GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization (FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene.

Synonyms

Glial cell line-derived neurotrophic factor;hGDNF;Astrocyte-derived trophic factor;ATF;GDNF;

Gene Name

Glial cell line-derived neurotrophic factor

UniProt

P39905

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of <a href="https://www.bosterbio.com/human-gdnf-picokine-trade-elisa-kit-ek0362-boster.html" style="color:#EA8D28">human GDNF</a>, different from the related mouse sequence by three amino acids and rat sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Iwashita, T., Kruger, G. M., Pardal, R., Kiel, M. J., Morrison, S. J.Hirschsprung disease is linked to defects in neural crest stem cell function.Science 301: 972-976, 2003. 2. Lin, L.-F. H., Doherty, D. H., Lile, J. D., Bektesh, S., Collins, F.GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons.Science 260: 1130-1132, 1993. 3. Schindelhauer, D., Schuffenhauer, S., Gasser, T., Steinkasserer, A., Meitinger, T.The gene coding for glial cell line derived neurotrophic factor (GDNF) maps to chromosome 5p12-p13.1.Genomics 28: 605-607, 1995.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.