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Anti-ATF5 Antibody Picoband®, PE Conjugate

Boster Bio Anti-ATF5 Antibody Picoband® catalog # A02792-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Activating transcription factor 5, also known as ATF5, is a protein that, in humans, is encoded by the ATF5 gene. Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II transcription regulatory region sequence-specific DNA binding activity; and tubulin binding activity. Involved in several processes, including fat cell differentiation; regulation of cell cycle process; and regulation of transcription, DNA-templated. Located in centrosome; cytosol; and nucleoplasm.

Synonyms

ATF5; ATFX; Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx

Gene Name

activating transcription factor 5

Gene ID

22809

UniProt

Q9Y2D1

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human ATF5 recombinant protein (Position: L11-R275). Human ATF5 shares 86.5% and 87.2% amino acid (aa) sequence identity with mouse and rat ATF5, respectively.

Clonality

Polyclonal

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Pati, D., Meistrich, M. L., Plon, S. E. Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis. Molec. Cell. Biol. 19: 5001-5013, 1999. 2. Peters, C. S., Liang, X., Li, S., Kannan, S., Peng, Y., Taub, R., Diamond, R. H. ATF-7, a novel bZIP protein, interacts with the PRL-1 protein-tyrosine phosphatase. J. Biol. Chem. 276: 13718-13726, 2001. 3. Sheng, Z., Li, L., Zhu, L. J., Smith, T. W., Demers, A., Ross, A. H., Moser, R. P., Green, M. R. A genome-wide RNA interference screen reveals an essential CREB3L2-ATF5-MCL1 survival pathway in malignant glioma with therapeutic implications. Nature Med. 16: 671-677, 2010.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

31 kDa

Observed Molecular Weight

37 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Protein Name

Cyclic AMP-dependent transcription factor ATF-5

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