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Anti-POLR3D Antibody Picoband®, PE Conjugate

Boster Bio Anti-POLR3D Antibody Picoband® catalog # A11611-2. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

DNA-ed RNA polymerase III subunit RPC4 is an enzyme that in humans is encoded by the POLR3D gene. This gene complements a temperature-sensitive mutant isolated from the BHK-21 Syrian hamster cell line. It leads to a block in progression through the G1 phase of the cell cycle at nonpermissive temperatures.

Gene Name

POLR3D

Gene ID

661

UniProt

P05423

Host

Rabbit

Reactivity

Human,Rat

Immunogen

E.coli-derived human POLR3D recombinant protein (Position: K231-L360). Human POLR3D shares 92.3% amino acid (aa) sequence identity with mouse POLR3D.

Clonality

Polyclonal

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Greco, A., Ittmann, M., Barletta, C., Basilico, C., Croce, C. M., Cannizzaro, L. A., Huebner, K. Chromosomal localization of human genes required for G(1) progression in mammalian cells. Genomics 4: 240-245, 1989. 2. Ittmann, M., Greco, A., Basilico, C. Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells. Molec. Cell. Biol. 7: 3386-3393, 1987.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

44 kDa

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

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