Anti-NAPB Antibody Picoband®, PE Conjugate

Boster Bio Anti-NAPB Antibody Picoband® catalog # A00939-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Beta-soluble NSF attachment protein is a SNAP protein involved in vesicular trafficking and exocytosis which is encoded by the NAPB gene humans is. This gene encodes a member of the soluble N-ethyl-maleimide-sensitive fusion attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. This gene encodes the SNAP beta isoform which has been shown to be preferentially expressed in brain tissue. The encoded protein also interacts with the GluR2 -amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit C-terminus and may play a role as a chaperone in the molecular processing of the AMPA receptor.

Synonyms

Tafazzin; Protein G4.5; TAZ; EFE2, G4.5

Gene Name

tafazzin

Gene ID

63908

UniProt

Q9H115

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminusof human NAPB, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

High levels in cardiac and skeletal muscle. Up to 10 isoforms can be present in different amounts in different tissues. Most isoforms are ubiquitous. Isoforms that lack the N-terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. Some forms appear restricted to cardiac and skeletal muscle or to leukocytes.

Applications

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Some isoforms may be involved in cardiolipin (CL) metabolism.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Burgalossi, A., Jung, S., Meyer, G., Jockusch, W. J., Jahn, O., Taschenberger, H., O'Connor, V. M., Nishiki, T., Takahashi, M., Brose, N., Rhee, J.-S. SNARE protein recycling by alpha-SNAP and beta-SNAP supports synaptic vesicle priming. Neuron 68: 473-487, 2010. Note: Erratum: Neuron 73: 620 only, 2012. 2. Conroy, J., Allen, N. M., Gorman, K. M., Shahwan, A., Ennis, S., Lynch, S. A., King, M. D. NAPB--a novel SNARE-associated protein for early-onset epileptic encephalopathy. Clin. Genet. 89: E1-E3, 2016. 3. Reuter, M. S., Tawamie, H., Buchert, R., Hosny Gebril, O., Froukh, T., Thiel, C., Uebe, S., Ekici, A. B., Krumbiegel, M., Zweier, C., Hoyer, J., Eberlein, K., and 17 others. Diagnostic yield and novel candidate genes by exome sequencing in 152 consanguineous families with neurodevelopmental disorders. JAMA Psychiat. 74: 293-299, 2017.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.