Anti-C21orf45/MIS18A Antibody Picoband®, PE Conjugate

Boster Bio Anti-C21orf45/MIS18A Antibody Picoband® catalog # A10791-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Protein Mis18-alpha is a protein that in humans is encoded by the MIS18A gene. C21orf45, also named as MIS18A, C21orf46 and FASP1, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. C21orf45 has dimer (~52 kDa) and heterodimer(~105 kDa) forms.

Synonyms

RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2; NOVA2; ANOVA; NOVA3

Gene Name

NOVA alternative splicing regulator 2

Gene ID

54069

UniProt

Q9NYP9

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human C21orf45/MIS18A recombinant protein (Position: M1-D233).

Clonality

Polyclonal

Tissue Specificity

Brain. Expression restricted to astrocytes.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May regulate RNA splicing or metabolism in a specific subset of developing neurons. Binds single strand RNA.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Fujita, Y., Hayashi, T., Kiyomitsu, T., Toyoda, Y., Kokubu, A., Obuse, C., Yanagida, M. Priming of centromere for CENP-A recruitment by human hMi18-alpha, hMis18-beta, and M18BP1. Dev. Cell 12: 17-30, 2007. 2. Gross, M. B. Personal Communication. Baltimore, Md. 9/28/2018. 3. Kim, I. S., Lee, M., Park, K. C., Jeon, Y., Park, J. H., Hwang, E. J., Jeon, T. I., Ko, S., Lee, H., Baek, S. H., Kim, K. I. Roles of Mis18-alpha in epigenetic regulation of centromeric chromatin and CENP-A loading. Molec. Cell 46: 260-273, 2012.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

29370 MW

Observed Molecular Weight

26 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -