Anti-Melan-A/MLANA Antibody Picoband®, PE Conjugate

Boster Bio Anti-Melan-A/MLANA Antibody Picoband® catalog # A02033-3. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

MLANA (Melan-A, also known as MART1; MART-1), located on 9p24.1, is a Protein Coding gene. The gene produces a 13157 Da protein composed of 118 amino acids. MLANA is a hydrophobic transmembrane protein without glycosylation sites highly enriched in early melanosomes. MLANA is expressed in skin and retinae melanocytes and the majority of melanoma tumors, but it is absent from other tissues and tumors. Diseases such as Melanoma In Congenital Melanocytic Nevus and Angiomyolipoma are associated with MLANA. The related pathways of MLANA include NF-kappaB Signaling.

Synonyms

Tripartite motif-containing protein 6; RING finger protein 89; RING-type E3 ubiquitin transferase TRIM6; TRIM6; RNF89

Gene Name

tripartite motif containing 6

Gene ID

2315

UniProt

Q16655

Host

Rabbit

Reactivity

Human,Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Melan-A/MLANA recombinant protein (Position: M1-L37).

Clonality

Polyclonal

Tissue Specificity

Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

E3 ubiquitin-protein ligase which ubiquitinates MYC and inhibits its transcription activation activity, maintaining the pluripotency of embryonic stem cells. Involved in the synthesis of unanchored K48-linked polyubiquitin chains which interact with and activate the serine/threonine kinase IKBKE, leading to phosphorylation of STAT1 and stimulation of an antiviral response .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Coulie, P. G., Brichard, V., Van Pel, A., Wolfel, T., Schneider, J., Traversari, C., Mattei, S., De Plaen, E., Lurquin, C., Szikora, J.-P., Renauld, J.-C., Boon, T. A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas. J. Exp. Med. 180: 35-42, 1994. 2. Giordano, F., Bonetti, C., Surace, E. M., Marigo, V., Raposo, G. The ocular albinism type 1 (OA1) G-protein-coupled receptor functions with MART-1 at early stages of melanogenesis to control melanosome identity and composition. Hum. Molec. Genet. 18: 4530-4545, 2009. 3. Gross, M. B. Personal Communication. Baltimore, Md. 10/30/2017.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.