Anti-MAGED4 Antibody Picoband®, PE Conjugate

Boster Bio Anti-MAGED4 Antibody Picoband® catalog # A16674-1. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

MAGE family member D4 is a protein that in humans is encoded by the MAGED4 gene. MAGED4, originally termedMAGEE1, is a member ofMAGE (Melanoma associated antigen)super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556; 24966945). Three isoforms(MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of humanMAGED4 protein detects a band of 90-100 kDa, which islargerthan the calculated molecularweight. The slower electrophoretic mobility may be due to abnormal spatial conformation ofMAGED4 protein caused by abundance of proline in composition (PMID: 24966945).

Synonyms

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

Gene Name

kelch repeat and BTB domain containing 2

Gene ID

728239

UniProt

Q96JG8

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MAGED4 recombinant protein (Position: F51-R741).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Chomez, P., De Backer, O., Bertrand, M., De Plaen, E., Boon, T., Lucas, S. An overview of the MAGE gene family with the identification of all human members of the family. Cancer Res. 61: 5544-5551, 2001. [PubMed: 11454705, related citations] 2. Hartz, P. A. Personal Communication. Baltimore, Md. 4/30/2009. 3. Ito, S., Kawano, Y., Katakura, H., Takenaka, K., Adachi, M., Sasaki, M., Shimizu, K., Ikenaka, K., Wada, H., Tanaka, F. Expression of MAGE-D4, a novel MAGE family antigen, is correlated with tumor-cell proliferation of non-small cell lung cancer. Lung Cancer 51: 79-88, 2006.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

24145 MW

Observed Molecular Weight

100 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry, 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -