Anti-NUBP1 Antibody Picoband®, PE Conjugate

Boster Bio Anti-NUBP1 Antibody Picoband® catalog # A08538-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

NUBP1 also known as NBP as is expressed in several tissues with the highest being in the lung and testis. NUBP1 appears to interact with actin and the cytoskeleton and is required for centrosome duplication but it is also need for proper synthesis of iron sulfur proteins and the protein also binds ATP. NUBP1 has roles in cell growth, cellular iron homeostasis, centrosome localization, iron-sulfur cluster assembly, and apolipoprotein modification. NUBP1 is localized to the cytoplasm. Interestingly too, recent research indicates that mutations in NUBP1 in mice lead defects in lung development and eye cataracts, many of which are traced back to NUBP1 role in regulating centrosome dynamics and microtubule organization.

Synonyms

PHD finger protein 21A; BHC80a; BRAF35-HDAC complex protein BHC80; PHF21A; BHC80; KIAA1696; BM-006

Gene Name

PHD finger protein 21A

Gene ID

4682

UniProt

P53384

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NUBP1 recombinant protein (Position: M1-Q311).

Clonality

Polyclonal

Tissue Specificity

Highly expressed in brain. Expressed at much lower level in other tissues.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 9/11/2012. 2. Nakashima, H., Grahovac, M. J., Mazzarella, R., Fujiwara, H., Kitchen, J. R., Threat, T. A., Ko, M. S. H. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to the chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes. Genomics 60: 152-160, 1999. 3. Shahrestanifar, M., Saha, D. P., Scala, L. A., Basu, A., Howells, R. D. Cloning of a human cDNA encoding a putative nucleotide-binding protein related to Escherichia coli MinD. Gene 147: 281-285, 1994.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

35 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -