Anti-MGAT2 Antibody Picoband®, PE Conjugate

Boster Bio Anti-MGAT2 Antibody Picoband® catalog # A06432-1. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase is an enzyme that in humans is encoded by the MGAT2 gene. The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined.

Synonyms

RasGAP-activating-like protein 1; RAS protein activator like 1

Gene Name

RAS protein activator like 1

Gene ID

4247

UniProt

Q10469

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MGAT2 recombinant protein (Position: Q81-K427).

Clonality

Polyclonal

Tissue Specificity

Highly expressed in thyroid and adrenal medulla, lower expression in brain, spinal cord and trachea (PubMed:9751798). Expressed in melanocytes (PubMed:23999003).

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Probable inhibitory regulator of the Ras-cyclic AMP pathway (PubMed:9751798). Plays a role in dendrite formation by melanocytes (PubMed:23999003).

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Alazami, A. M., Monies, D., Meyer, B. F., Alzahrani, F., Hashem, M., Salih, M. A., Alkuraya, F. S. Congenital disorder of glycosylation IIa: the trouble with diagnosing a dysmorphic inborn error of metabolism. (Letter) Am. J. Med. Genet. 158A: 245-246, 2012. 2. Alkuraya, F. S. Mental retardation, growth retardation, unusual nose, and open mouth: an autosomal recessive entity. Am. J. Med. Genet. 152A: 2160-2163, 2010. 3. Cormier-Daire, V., Amiel, J., Vuillaumier-Barrot, S., Tan, J., Durand, G., Munnich, A., Le Merrer, M., Seta, N. Congenital disorders of glycosylation IIa cause growth retardation, mental retardation, and facial dysmorphism. J. Med. Genet. 37: 875-877, 2000.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

50 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -