Anti-MED12 Antibody Picoband®, PE Conjugate

Boster Bio Anti-MED12 Antibody Picoband® catalog # A00828-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae), also known as MED12, is a human gene found on the X chromosome. The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.

Synonyms

Protein argonaute-1;Argonaute1;hAgo1;Argonaute RISC catalytic component 1;Eukaryotic translation initiation factor 2C 1;eIF-2C 1;eIF2C 1;Putative RNA-binding protein Q99;AGO1;EIF2C1;

Gene Name

Protein argonaute-1

Gene ID

9968

UniProt

Q93074

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human MED12 recombinant protein (Position: Q752-Q1974).

Clonality

Polyclonal

Tissue Specificity

Detected in blood plasma (at protein level).

Applications

WB,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Barbieri, C. E., Baca, S. C., Lawrence, M. S., Demichelis, F., Blattner, M., Theurillat, J.-P., White, T. A., Stojanov, P., Van Allen, E., Stransky, N., Nickerson, E., Chae, S.-S., and 34 others. Exome sequencing identifies recurrent SPOP, FOXA1 and MED12 mutations in prostate cancer. Nature Genet. 44: 685-689, 2012. 2. Beyer, K. S., Klauck, S. M., Benner, A., Poustka, F., Poustka, A. Association studies of the HOPA dodecamer duplication variant in different subtypes of autism. Am. J. Med. Genet. 114: 110-115, 2002. 3. Cools, F., Jaeken, J. Hardikar syndrome: a new syndrome with cleft lip/palate, pigmentary retinopathy and cholestasis. Am. J. Med. Genet. 71: 472-474, 1997.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.