Anti-NOSIP Antibody Picoband®, PE Conjugate

Boster Bio Anti-NOSIP Antibody Picoband® catalog # A07558-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nitric oxide synthase-interacting protein is an enzyme that in humans is encoded by the NOSIP gene. The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein.

Synonyms

C-C motif chemokine 16; Chemokine CC-4; HCC-4; Chemokine LEC; IL-10-inducible chemokine; LCC-1; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; LMC; Monotactin-1; MTN-1; NCC-4; Small-inducible cytokine A16; CCL16; ILINCK; NCC4; SCYA16

Gene Name

C-C motif chemokine ligand 16

Gene ID

51070

UniProt

Q9Y314

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NOSIP recombinant protein (Position: M1-R278).

Clonality

Polyclonal

Tissue Specificity

Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

Applications

ELISA,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Dedio, J., Konig, P., Wohlfart, P., Schroeder, C., Kummer, W., Muller-Esterl, W. NOSIP, a novel modulator of endothelial nitric oxide synthase activity. FASEB J. 15: 79-89, 2001. 2. Hoffmeister, M., Prelle, C., Kuchler, P., Kovacevic, I., Moser, M., Muller-Esterl, W., Oess, S. The ubiquitin E3 ligase NOSIP modulates protein phosphatase 2A activity in craniofacial development. PLoS One 9: e116150, 2014. Note: Electronic Article.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.