Anti-NFKBIZ Antibody Picoband®, PE Conjugate

Boster Bio Anti-NFKBIZ Antibody Picoband® catalog # A05312-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

NF-kappa-B inhibitor zeta (IκBζ) is a protein that in humans is encoded by the NFKBIZ gene.This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Loricrin; LOR; LRN

Gene Name

loricrin

Gene ID

64332

UniProt

Q9BYH8

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NFKBIZ recombinant protein (Position: Q247-H577).

Clonality

Polyclonal

Tissue Specificity

Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

Applications

ELISA,IF,ICC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Major keratinocyte cell envelope protein.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Bambouskova, M., Gorvel, L., Lampropoulou, V., Sergushichev, A., Loginicheva, E., Johnson, K., Korenfeld, D., Mathyer, M. E., Kim, H., Huang, L.-H., Duncan, D., Bregman, H., and 19 others. Electrophilic properties of itaconate and derivatives regulate the I-kappa-B-zeta-ATF3 inflammatory axis. Nature 556: 501-504, 2018. 2. Eto, A., Muta, T., Yamazaki, S., Takeshige, K. Essential roles for NF-kappa-B and a Toll/IL-1 receptor domain-specific signal(s) in the induction of I-kappa-B-zeta. Biochem. Biophys. Res. Commun. 301: 495-501, 2003. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/19/2020.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

85 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -