Anti-SDCCAG1/NEMF Antibody Picoband®, PE Conjugate

Boster Bio Anti-SDCCAG1/NEMF Antibody Picoband® catalog # A09039-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nuclear export mediator factor (NEMF) is a protein that in humans is encoded by the NEMF gene. This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor.

Synonyms

Tudor domain-containing protein 3; TDRD3

Gene Name

tudor domain containing 3

Gene ID

9147

UniProt

O60524

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SDCCAG1/NEMF recombinant protein (Position: H81-R607).

Clonality

Polyclonal

Tissue Specificity

Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Applications

WB,ICC,IF,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ahmed, A., Wang, M., Bergant, G., Maroofian, R., Zhao, R., Alfadhel, M., Nashabat, M., AlRifai, M. T., Eyaid, W., Alswaid, A., Beetz, C., Qin, Y., and 21 others. Biallelic loss-of-function variants in NEMF cause central nervous system impairment and axonal polyneuropathy. Hum. Genet. 140: 579-592, 2021. 2. Anazi, S., Maddirevula, S., Faqeih, E., Alsedairy, H., Alzahrani, F., Shamseldin, H. E., Patel, N., Hashem, M., Ibrahim, N., Abdulwahab, F., Ewida, N., Alsaif, H. S., and 36 others. Clinical genomics expands the morbid genome of intellectual disability and offers a high diagnostic yield. Molec. Psychiat. 22: 615-624, 2017. 3. Carbonnelle, D., Liehr, T., Jacquot, C., Masson, D., Lustenberger, P., Denis, M. G., Roussakis, C. Assignment of the serologically defined colon cancer antigen 1 gene (SDCCAG1) to human chromosome band 14q22 by in situ hybridization. Cytogenet. Cell Genet. 86: 248-249, 1999.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.