Anti-NECAB1 Antibody Picoband®, PE Conjugate

Boster Bio Anti-NECAB1 Antibody Picoband® catalog # A13973-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

NECAB1, also named as EFCBP1, is a brain-specifically expressed gene with highest abundance in temporal lobe, encodes a protein containing EF-hand and antibiotic biosynthesis monooxygenase domains Short Communication.

Synonyms

RNA-binding protein 47;RNA-binding motif protein 47;RBM47;

Gene Name

RNA-binding protein 47

Gene ID

64168

UniProt

Q8N987

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human NECAB1 recombinant protein (Position: E16-E296).

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

WB,IHC,ICC,IF,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Wu, H. , Li, D. , Shan, Y. , Wan, B. , Hexige, S. , & Guo, J. , et al. (2007). Efcbp1/necab1, a brain-specifically expressed gene with highest abundance in temporal lobe, encodes a protein containing ef-hand and antibiotic biosynthesis monooxygenase domains. Dna Seq, 18(1), 73-79. 2. Zhang, M. D. , Barde, S. , Szodorai, E. , Josephson, A. , Mitsios, N. , & Watanabe, M. , et al. (2016). Comparative anatomical distribution of neuronal calcium-binding protein (necab) 1 and -2 in rodent and human spinal cord. Brain Structure & Function, 221(7), 1-21. 3. Sugita, S. , Ho, A. , & T.C. Südhof. (2002). Necabs: a family of neuronal ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern. Neuroscience, 112(1), 51-63.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64099 MW

Observed Molecular Weight

41 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>