Anti-NDUFA4 Antibody Picoband®, PE Conjugate

Boster Bio Anti-NDUFA4 Antibody Picoband® catalog # A09276-3. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

NDUFA4, mitochondrial complex associated is a protein that in humans is encoded by the NDUFA4 gene. The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Synonyms

Interleukin 1 family member 10 ;Interleukin 1 family, member 10 (Predicted) ;Il1f10 ;Il1f10_predicted ;rCG_45898 ;

Gene Name

interleukin 1 family member 10 (theta)

Gene ID

4697

UniProt

O00483

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NDUFA4 recombinant protein (Position: M1-F81).

Clonality

Polyclonal

Tissue Specificity

Expressed in fetal skin, spleen and tonsil. Expressed mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS). Ligand for IL-36R/IL1RL2.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Zhang, Y. , Ge, M. , Chen, Y. , Yang, Y. , Chen, W. , & Wu, D. , et al. (2022). Ndufa4 promotes cell proliferation by enhancing oxidative phosphorylation in pancreatic adenocarcinoma. Journal of Bioenergetics and Biomembranes, 54(5), 283-291. 2. Xu, W. , Lai, Y. , Pan, Y. , Tan, M. , Ma, Y. , & Sheng, H. , et al. M6a rna methylation-mediated ndufa4 promotes cell proliferation and metabolism in gastric cancer. Cell Death & Disease. 3. Ramzan, R. , Rhiel, A. , Weber, P. , Kadenbach, B. , & Vogt, S. . (2019). Reversible dimerization of cytochrome c oxidase regulates mitochondrial respiration. Mitochondrion, 49.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

17081 MW

Observed Molecular Weight

12 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -