Anti-ROBO4 Antibody Picoband®, PE Conjugate

Boster Bio Anti-ROBO4 Antibody Picoband® catalog # A04479-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Roundabout homolog 4 is a protein that in humans is encoded by the ROBO4 gene. Predicted to enable cell-cell adhesion mediator activity. Involved in angiogenesis and establishment of endothelial barrier. Located in extracellular exosome. Implicated in aortic valve disease 3.

Synonyms

Integrin alpha-L;CD11 antigen-like family member A;Leukocyte adhesion glycoprotein LFA-1 alpha chain;LFA-1A;Leukocyte function-associated molecule 1 alpha chain;CD11a;ITGAL;CD11A;

Gene Name

Integrin alpha-L

Gene ID

54538

UniProt

Q8WZ75

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ROBO4 recombinant protein (Position: D108-A933).

Clonality

Polyclonal

Tissue Specificity

Leukocytes.

Applications

WB,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Bedell, V. M., Yeo, S.-Y., Park, K. W., Chung, J., Seth, P., Shivalingappa, V., Zhao, J., Obara, T., Sukhatme, V. P., Drummond, I. A., Li, D. Y., Ramchandran, R. roundabout4 is essential for angiogenesis in vivo. Proc. Nat. Acad. Sci. 102: 6373-6378, 2005. 2. Gould, R. A., Aziz, H., Woods, C. E., Seman-Senderos, M. A., Sparks, E., Preuss, C., Wunnemann, F., Bedja, D., Moats, C. R., McClymont, S. A., Rose, R., Sobreira, N., and 33 others. ROBO4 variants predispose individuals to bicuspid aortic valve and thoracic aortic aneurysm. Nature Genet. 51: 42-50, 2019. 3. Huminiecki, L., Gorn, M., Suchting, S., Poulsom, R., Bicknell, R. Magic roundabout is a new member of the roundabout receptor family that is endothelial specific and expressed at sites of active angiogenesis. Genomics 79: 547-552, 2002.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.