Anti-PRUNE1 Antibody Picoband®, PE Conjugate

Boster Bio Anti-PRUNE1 Antibody Picoband® catalog # A31798. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants.

Synonyms

Protein Bop; BH3-only protein; Retrotransposon Gag-like protein 10; RTL10; BOP, C22orf29

Gene Name

retrotransposon Gag like 10

Gene ID

58497

UniProt

Q86TP1

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PRUNE1 recombinant protein (Position: M1-Q444).

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Could induce apoptosis in a BH3 domain-dependent manner. The interaction network of Bcl-2 family members may play a key role in modulation RTL10/BOP intrinsic apoptotic signaling activity.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Alhaddad, B., Schossig, A., Haack, T. B., Kovacs-Nagy, R., Braunisch, M. C., Makowski, C., Senderek, J., Vill, K., Muller-Felber, W., Strom, T. M., Krabichler, B., Freisinger, P., and 17 others. PRUNE1 deficiency: expanding the clinical and genetic spectrum. Neuropediatrics 49: 330-338, 2018. 2. Costain, G., Shugar, A., Krishnan, P., Mahmutoglu, S., Laughlin, S., Kannu, P. Homozygous mutation in PRUNE1 in an Oji-Cree male with a complex neurological phenotype. Am. J. Med. Genet. 173A: 740-743, 2017. 3. Diana, D., Smaldone, G., De Antonellis, P., Pirone, L., Carotenuto, M., Alonzi, A., Di Gaetano, S., Zollo, M., Pedone, E. M., Fattorusso, R. Mapping functional interaction sites of human prune C-terminal domain by NMR spectroscopy in human cell lysates. Chemistry 19: 12217-12220, 2013.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

14377 MW

Observed Molecular Weight

60 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -