Anti-SEMA3D Antibody Picoband®, PE Conjugate

Boster Bio Anti-SEMA3D Antibody Picoband® catalog # A09239-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin like domain and a C-terminal basic domain. The protein encoded by this gene binds neuropilin and plays an important role in cardiovascular development.

Synonyms

Nck-associated protein 1;NAP 1;Membrane-associated protein HEM-2;p125Nap1;NCKAP1;HEM2, KIAA0587, NAP1;

Gene Name

Nck-associated protein 1

Gene ID

223117

UniProt

O95025

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SEMA3D recombinant protein (Position: D88-H697).

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.

Applications

WB,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Jin, Z., Chau, M. D., Bao, Z.-Z. Sema3D, Sema3F, and Sema5A are expressed in overlapping and distinct patterns in chick embryonic heart. Dev. Dyn. 235: 163-169, 2006. 2. Liu, Y., Halloran, M. C. Central and peripheral axon branches from one neuron are guided differentially by semaphorin3D and transient axonal glycoprotein-1. J. Neurosci. 25: 10556-10563, 2005.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

128790 MW

Observed Molecular Weight

90 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cell membrane ; Single-pass membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Single-pass membrane protein ; Cytoplasmic side . At the interface between the lamellipodial actin meshwork and the membrane. .