Anti-Angiogenin/Ang Antibody Picoband®, PE Conjugate

Boster Bio Anti-Angiogenin/Ang Antibody Picoband® catalog # A00146-3. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Angiogenin (ANG) also known as ribonuclease 5 is a small 123 amino acid protein that in humans is encoded by the ANG gene. Predicted to enable several functions, including actin binding activity; heparin binding activity; and protein homodimerization activity. Involved in liver development. Predicted to be located in several cellular components, including growth cone; neuronal cell body; and nucleolus. Predicted to be part of angiogenin-PRI complex. Human ortholog(s) of this gene implicated in diabetic retinopathy; myocardial infarction; neurodegenerative disease (multiple); and prostate cancer. Orthologous to human ANG (angiogenin).

Synonyms

Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5;

Gene Name

Angiogenin

Gene ID

305843

UniProt

Q5WRG2

Host

Rabbit

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived rat Angiogenin/Ang recombinant protein (Position: Q25-L145).

Clonality

Polyclonal

Tissue Specificity

Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. .

Applications

WB,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Brown, W. E., Nobile, V., Subramanian, V., Shapiro, R. The mouse angiogenin gene family: structures of an angiogenin-related protein gene and two pseudogenes. Genomics 29: 200-206, 1995. 2. Crabtree, B., Thiyagarajan, N., Prior, S. H., Wilson, P., Iyer, S., Ferns, T., Shapiro, R., Brew, K., Subramanian, V., Acharya, K. R. Characterization of human angiogenin variants implicated in amyotrophic lateral sclerosis. Biochemistry 46: 11810-11818, 2007. 3. Gao, X., Hu, H., Zhu, J., Xu, Z. Identification and characterization of follistatin as a novel angiogenin-binding protein. FEBS Lett. 581: 5505-5510, 2007.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

16550 MW

Observed Molecular Weight

25 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Rat<br> ELISA, 0.1-0.5 μg/ml, Rat<br>

Subcellular Location

Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.