Anti-CD326/Epcam Antibody Picoband®, PE Conjugate

Boster Bio <a href="https://www.bosterbio.com/anti-ep-cam-cd326-epithelial-marker-antibody-m30941-boster.html">Anti-CD326/Epcam Antibody</a> Picoband® catalog # A00276-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Synonyms

Agouti-related protein; Agrp; Agrt; Art

Gene Name

agouti related neuropeptide

Gene ID

17075

UniProt

Q99JW5

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse CD326/Epcam recombinant protein (Position: Q24-A267).

Clonality

Polyclonal

Tissue Specificity

Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.

Applications

ELISA,Flow Cytometry,IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity (By similarity). It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner (By similarity).

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Litvinov, Sergey; et al. (1994). "Ep-CAM: a human epithelial antigen is a homophilic cell–cell adhesion molecule". The Journal of Cell Biology 125 (2): 437–46. 2. Maetzel, Dorothea; et al. (2009). "Nuclear signalling by tumour-associated antigen EpCAM". Nature Cell Biology 11 (2): 162–71.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

68762 MW

Observed Molecular Weight

38 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Mouse Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Mouse, Rat Immunofluorescence, 5 μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Mouse ELISA, 0.1-0.5 μg/ml, -