Anti-OLAH Antibody Picoband®, PE Conjugate

Boster Bio Anti-Anti-OLAH Antibody Picoband® Antibody Picoband® catalog # A05069-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Enables dodecanoyl-[acyl-carrier-protein] hydrolase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; and palmitoyl-[acyl-carrier-protein] hydrolase activity. Involved in medium-chain fatty acid biosynthetic process. Predicted to be located in cytosol.

Synonyms

Matrix metalloproteinase-16; MMP-16; 3.4.24.-; MMP-X2; Membrane-type matrix metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 matrix metalloproteinase; MT3-MMP; MT3MMP; MMP16; MMPX2;

Gene Name

matrix metallopeptidase 16

Gene ID

55301

UniProt

Q9NV23

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human OLAH recombinant protein (Position: E13-F265).

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Applications

ELISA,Flow Cytometry,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ritchie, M. K. , Johnson, L. C. , Clodfelter, J. E. , Pemble, C. W. , Fulp, B. E. , & Furdui, C. M. , et al. (2015). Crystal structure and substrate specificity of human thioesterase 2: insights into the molecular basis for the modulation of fatty acid synthase. Journal of Biological Chemistry, 3520-3530. 2. Nobuo, N. , Yasunori, S. , Takahiro, T. , Hiroaki, O. , Daisuke, O. , & Hanjun, Z. , et al. (2006). Isolation and expression profiling of genes upregulated in bone marrow-derived mononuclear cells of rheumatoid arthritis patients. Dna Research An International Journal for Rapid Publication of Reports on Genes & Genomes, 13(4), 169. 3. Grupe, A. , Li, Y. , Rowland, C. , Nowotny, P. , Hinrichs, A. L. , & Smemo, S. , et al. (2006). A scan of chromosome 10 identifies a novel locus showing strong association with late-onset alzheimer disease. The American Journal of Human Genetics, 78(1), 78-88.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39125 MW

Observed Molecular Weight

36 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -